Antibacterial activity of bile on Escherichia coli and Salmonella spp isolated from faeces, urine and blood.
Abstract
The antibacterial activity of bile on Escherichia coli and Salmonella spp isolated from faeces,
urine and blood was determined in this study. Bile from three animal origin (cow ,goat and
chicken) was extracted using alcohol and water and their antibacterial activity tested using the
agar well diffusion method at different concentrations . The crude extracts exerted the highest
inhibition zone diameter within the range of 20mm-49mm and 38mm-48mm against Salmonella
spp and Escherichia coli respectively, followed by the alcoholic extract with inhibition zone
diameter in the range of 6mm-35mm and 7mm-33mm against Salmonella spp and Escherichia
coli respectively. While the aqueous extract exhibited the least activity in the range of 5mm-
25mm and 7mm- 25mm against Salmonella spp and Escherichia coli respectively. The MIC of
the bile extracts which was determined using the tube macrodilution method and the MBC
showed that bile have both bactericidal and bacteriostatic activity on the test organisms. The
findings of this work has shown that bile contains some substances that can exert inhibitory
effect on bacteria hence can be employed in the treatment of diseases caused by these groups of
bacteria.
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